Afterwards, place the sample tubes in a centrifuge to separate the lysate. Tune the extent of disruption by adjusting the run time and speed. Simply place your sample, beads and buffer in standard tubes, and load the tubes into the Bullet Blender. The Bullet Blender Gold is designed for reliability and durability, with few moving parts and a very low rate of motor wear. It uses a DC motor to agitate the individual tubes. The instrument uses little power due to the unique, patented design, so there is very little heat build up in the instrument or inside the sample tubes. The Bullet Blender Gold uses dry ice for easy, inexpensive, maintenance-free cooling that keeps samples at 4☌ during processing. The sample tubes are kept closed and self-contained during homogenization. There are no probes or blades to cause cross contamination or sample loss, which also means there are no parts to clean between runs. Consistent ResultsĪll of the samples are automatically homogenized in the same manner to minimize sample-to-sample variability. There is also a standard model which homogenizes up to 24 small samples (up to 300 mg) at a time in standard 1.5 ml polypropylene snap-cap microcentrifuge tubes or the mid-sized "5" model which homogenizes up to 12 mid-sized samples (up to 1g) at a time in 5 mL Eppendorf® snap-cap tubes. This "deluxe" size Bullet Blender Gold homogenizes up to 8 large samples (up to 3.5 g) at a time in standard 50 ml skirted polypropylene centrifuge tubes. The air circulation will adjust according to homogenization conditions, allowing the contents of the tube to reach and maintain a temperature of 4☌. The 4☌ signal will light up when the temperature is reached. The Bullet Blender Gold includes dry ice cooling to keep samples at or near 4☌, have built-in sound insulation for quieter operation, and an upgraded motor for more efficient homogenization of tougher samples as well as faster homogenization of soft ones! Fill the side compartment with dry ice and cold air will circulate through the inside of the instrument. The "Gold" models of the Bullet Blenders include a number of upgrades. Load the samples into the tubes, place them in the Bullet Blender, set the time and speed and you're ready! The "bullets" in the instrument vigorously strike all of the tubes and homogenize your sample in minutes. The Bullet Blender® 50 Gold enables you to homogenize, disrupt, or lyse up to 8 large tissue or cell culture samples (up to 3 mg solid weight, 10-15 ml total volume) at a time in standard polypropylene tubes, all while keeping them at refrigerated temperatures near 4☌.
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